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Cell cycle analysis in a human cell line (EUE cells)

✍ Scribed by R. Zippel; E. Martegani; M. Vanoni; G. Mazzini; Prof. L. Alberghina

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
482 KB
Volume
2
Category
Article
ISSN
0196-4763

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✦ Synopsis

Abstract

A new procedure to determine the durations of cell cycle phases is based on the autoradiography of labeled nuclei and a standard chemical determination of DNA content per cell. It has been used to study the cell cycle timing of a human established cell line, EUE cells, so far poorly characterized in its kinetic aspects. The cycle phase durations obtained with this method are compared with the DNA frequency profiles determined by flow cytometry and found to agree closely. The relevance of the growth conditions of the exponentially growing cultures for a correct estimation of the cycle phase duration is indicated. Cell size distribution has been shown to vary according to growth condition.

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