An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o
✦ LIBER ✦
Cation binding linked to a sequence-specific CAP–DNA interaction
✍ Scribed by Douglas F. Stickle; Michael G. Fried
- Book ID
- 108074378
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 606 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0301-4622
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The interaction specificities of Co(III) with DNA were investigated via consideration of thermodynamic characteristics of the duplex to single strand transition for DNA oligomers incubated in the presence of [Co(NH 3 ) 5 (OH 2 )](ClO 4 ) 3 . It has previously been demonstrated that incubation of the