Carrier screening and prenatal gene diagnosis of β-thalassemia by PCR-RDB technique
✍ Scribed by Zhang Hongxiu; Shan Keren; Hui Chunlin; He Yan; Yuan Zhuhua; Dou Youlian; Zeng Jinlin; Xie Yuan; Xiu Jin
- Publisher
- Springer-Verlag
- Year
- 2003
- Tongue
- Chinese
- Weight
- 165 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0510-9752
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Alpha-thalassemia of Southeast Asian deletion (--SEA/) is very common in Southeast Asia. Homozygosity of this genotype is the major cause of Hb Bart's hydrops fetalis in Taiwan. With polymerase chain reaction using three oligonucleotide primers bridging the common deletion breakpoint, a DNA fragment
Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing beta-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacryla