✦ LIBER ✦

Detection of β-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique

✍ Scribed by Pranee Winichagoon; Jiraporn Kownkon; Pathai Yenchitsomanus; Varaporn Thonglairoam; Nopadol Siritanaratkul; Suthat Fucharoen

Publisher: Springer
Year: 1989
Tongue: English
Weight: 222 KB
Volume: 82
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00274004

No coin nor oath required. For personal study only.

✦ Synopsis

Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing beta-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG----TAG) and Hb E (GAG----AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively.

📜 SIMILAR VOLUMES

Hemoglobin E and codon 17 nonsense: Two

Hemoglobin E and codon 17 nonsense: Two β-globin gene mutations common in Southeast Asia detected by the use of ARMS

✍ H. Steger; A. Eigel; G. Flatz; J. Horst 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 293 KB

Detection of DNA amplification in 17 pri

Detection of DNA amplification in 17 primary breast carcinomas with homogeneously staining regions by a modified comparative genomic hybridization technique

✍ Martine Muleris; Anna Almeida; Michèle Gerbault-Seureau; Bernard Malfoy; Bernard 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 949 KB

## Abstract A modified comparative genomic hybridization (mCGH) technique was applied to a series of 17 primary breast carcinomas in which cytogenetic study (CG) demonstrated the presence of homogeneously staining region(s), suggesting the occurrence of DNA amplification. mCGH demonstrated recurren