Carbon-13 NMR spectroscopy of biological systems

✍ Scribed by Glickson, Jerry D. ;Opella, Stanley J.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 75 KB
• Volume: 10
• Category: Article
• ISSN: 1043-7347
• DOI: 10.1002/(sici)1099-0534(1998)10:2<129::aid-cmr4>3.0.co;2-z

✦ Synopsis

This book provides both an introduction for the novice and a review for the expert to the rapidly developing field of 13 C NMR spectroscopy of biological systems. It consists of a forward by Joachim Seelig, a preface by the author, and six chapters dealing with basic principles and key applications. The mix of theory and application defines the unique quality of this book.

Chapter 1, by Nicolau Beckmann, provides a Ž . brief summary 5 pages, 26 references of basic principles from a historical perspective. The reader is referred to basic texts for details, and landmark developments are cited. Beginning with continuous wave NMR spectroscopy, it describes the introduction of Fourier transform and double resonance techniques. Highlights of solid state NMR include polarization transfer, decoupling, and magic-angle spinning methods. The chapter ends with a description of heteronuclear polarization transfer, indirect proton NMR detection of 13 C, and spectral editing techniques.

Chapter 2, Gemmeker and Kessler, is unique in providing a coherent view of the multidimensional NMR methods applied to proteins and nucleic acids by structural biologists and the analytical NMR methods used by organic chemists. The success in bridging this gap makes this an extraordinarily valuable contribution; perhaps these authors are the only ones with sufficient experience to show that these two branches of NMR spectroscopy are not nearly as disparate as they may seem. The wide range of individual experiments are unified by the focus on the 13 C nucleus, which in most applications is observed through the nearby 1 H nuclei, leading naturally to multidimensional experiments that display the effects of the available chemical shift and spin coupling interactions. The analytical applications emphasize those experiments that are performed Ž .

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