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Capillary zone electrophoresis: Effect of physical parameters on separation efficiency and quantitation

✍ Scribed by Lukacs, K. D. ;Jorgenson, J. W.

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
431 KB
Volume
8
Category
Article
ISSN
0935-6304

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✦ Synopsis

Separation efficiency in capillary zone electrophoresis was examined as a function of capillary length and diameter as well as solute concentration for a borosilicate glass capillary/ phosphate buffer system. Electroosmotic flow coefficients in borosilicate, fused silica, and teflon capillaries were measured over the useful operating pH range of 3-8 at constant applied power (0.333 W). Sample introduction by a simple, lowdispersion electromigration method was found to be a reliable procedure for solute quantitation.

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