Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis
✍ Scribed by Reine Nehmé; Catherine Perrin; Hervé Cottet; Marie D. Blanchin; Huguette Fabre
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 442 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
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The capillary electrophoresis (CE) running electrolyte composition was optimized for the separation of selected glycoproteins. A good separation of the ovalbumin (OV) and alpha-acid glycoprotein (AAG) isoforms was achieved in 20 mmol x L(-1) N-(2-hydroxyethyl)piperazine-2'-(2-ethanesulfonic acid) (H
## Abstract The study was undertaken to improve the separation and the concentration sensitivity of catecholamines by capillary electrophoresis (CE) using laboratory‐coated capillaries. The coatings were made by adsorption using in‐run modification and by covalent bonding. The coating solutions use