Dependence of analyte separation on electroosmotic flow in capillary zone electrophoresis: Quantitative description by the reduced mobility

✍ Scribed by Ernst Kenndler

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 112 KB
• Volume: 10
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1998)10:3<273::aid-mcs6>3.0.co;2-d

✦ Synopsis

It is shown that the dimensionless reduced mobility, , which is the i ratio of the effective mobility, u , to the total mobility, u q u , of separand i eff, i eff, i eo Ž . defined as s u r u q u is that variable which allows to express the i eff, i eff, i eo Ž . Ž . effect of the electroosmotic flow EOF with mobility u on all separation eo parameters relevant for capillary zone electrophoresis quantitatively for nearly all conditions. Efficiency, selectivity, and resolution are expressed as a function of the reduced mobility. Based on this concept the influence of a certain EOF on the separation of cationic and anionic separands with different effective mobilities is discussed in detail. It is demonstrated that for cations the resolution is always Ž . reduced due to the EOF if directed toward the cathode as usual , whereas for anions with effective mobilities larger than u r2 the resolution is enhanced, eo compared to the case without EOF.