Capillary electrophoretic determination of triamterene, methotrexate, and creatinine in human urine

✍ Scribed by Juana Rodríguez Flores; Juan J. Berzas Nevado; Isabel Durán Merás; Maria J. Rodríguez Gómez

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 476 KB
• Volume: 28
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200400059

✦ Synopsis

Abstract

A capillary zone electrophoresis (CZE) method using a fused‐silica capillary (60.2 cm×75 μm ID) was investigated for the determination of triamterene (TRI), methotrexate (MTX), and creatinine (CREA) in human urine. The separation was performed using a hydrodynamic injection time of 7 s (0.5 psi), a voltage of 25 kV, a capillary temperature of 30°C, and 40 mM phosphoric acid adjusted to pH 2.25 by addition of triethanolamine as separation electrolyte. Under these conditions, analysis takes about 15 min. A linear response over the 0.5–15.0 mg L^–1^ concentration range was found for TRI and MTX, and 0.5–80.0 mg L^–1^ for CREA. Dilution of the sample (water:urine, 1 : 1 for TRI and MTX, and 1:25 for CREA determination) was the only step necessary prior to analysis by electrophoresis. The developed method is easy, rapid, and sensitive and has been applied to determine triamterene, methotrexate, and creatinine in urine samples with satisfactory results.