✍ Scribed by Maria Augusta Raggi; Roberto Mandrioli; Cesare Sabbioni; Francesca Mongiello; Marina Marini; Salvatore Fanali
No coin nor oath required. For personal study only.
A high-performance capillary electrophoretic method is described which Ž . allows for a sensitive and accurate determination of reduced glutathione GSH in human lymphocytes. The method makes use of a fused silica capillary with Ž polyacrylamide coating total length 27 cm, effective length 20 cm, 75 or 50 m . i.d. . The detection wavelength is 200 nm. The procedure is very simple: the lymphocyte lysate, after suitable filtration, is diluted 1:1 with pH 7.0 phosphate buffer and Ellman 's reagent is subsequently added. This reagent forms a stable adduct with GSH which blocks glutathione into its reduced form and enhances the w Ž .
x method sensitivity limit of detection LOD s 5 = 10 M . Reagent excess does not interfere and thus does not need to be eliminated. The method has the advantage of allowing for a rapid and simultaneous determination of both GSH Ž . and its oxidized form, glutathione disulfide GSSG , in a complex matrix such as that of human lymphocytes as well as of requiring only small volumes of the sample.
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## Abstract A capillary zone electrophoresis (CZE) method using a fused‐silica capillary (60.2 cm×75 μm ID) was investigated for the determination of triamterene (TRI), methotrexate (MTX), and creatinine (CREA) in human urine. The separation was performed using a hydrodynamic injection time of 7 s