Capillary electrophoresis of human serum proteins and apolipoproteins
✍ Scribed by Rainer Lehmann; Hartmut Liebich; Gerald Grübler; Prof. Dr. Dr. h.c. Wolfgang Voelter
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 361 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Capillary electrophoresis (CE) analysis of human serum proteins and apolipoproteins is described and compared with sodium dodecyl sulfate‐polyacryl‐amide gel electrophoresis and cellulose acetate membrane electrophoresis. Under optimized CE conditions apolipoproteins I could be determined directly in the serum and quantitated with a linear correlation between peak area and concentration up to a concentration of 100 mg/dL.
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## Abstract The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electro
Capillary gel electrophoresis was performed on a coated capillary column filled with a replaceable low-viscosity polymer network containing sodium dodecyl sulfate (eCAP SDS-200 kit from Beckman Instruments). While apolipoprotein (apo) A-I gave an homogeneous peak in high-density lipoprotein (HDL), i