Capillary Gel Electrophoresis Analysis of Apolipoproteins A-I and A-II in Human High-Density Lipoproteins

✍ Scribed by A. Goux; A. Athias; L. Persegol; L. Lagrost; P. Gambert; C. Lallemant

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 490 KB
• Volume: 218
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1185

✦ Synopsis

Capillary gel electrophoresis was performed on a coated capillary column filled with a replaceable low-viscosity polymer network containing sodium dodecyl sulfate (eCAP SDS-200 kit from Beckman Instruments). While apolipoprotein (apo) A-I gave an homogeneous peak in high-density lipoprotein (HDL), it appeared heterogeneous in its purified form. Apo A-II was heterogeneous in HDL as well as in purified preparations. For both proteins the relationship between peak areas and apo concentrations was linear over a large range of concentrations and, with the use of alpha-chymotrypsinogen A as an internal standard, apo A-I and apo A-II areas were measured with good precision (coefficient of variation 1.6 and 1.8%, respectively). Capillary gel electrophoresis appeared as a method of high-resolution power in the study of apo A-I and apo A-II heterogeneity and could lead to the development of an alternative method for the assay of apo HDL.