Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis

✍ Scribed by Atsushi Taga; Yuka Yamamoto; Rie Maruyama; Susumu Honda

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 109 KB
• Volume: 25
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200305765

✦ Synopsis

Abstract

The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electrophoresed in a neutral running buffer, containing human serum. Gen, the most significantly retarded disaccharide, was converted to its negatively charged bis‐mercaptoethanesulfonate derivative (MerESGen), and a serum sample was analyzed in a neutral buffer containing the derivatized disaccharide. Two peaks, belonging to the β‐globulin fraction, were found to be remarkably retarded in the buffer containing MerES‐Gen in a concentration‐dependent way. These findings prove an interaction between disaccharides and serum proteins.