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Capillary electrophoresis of DNA restriction fragments with solutions of entangled polymers

✍ Scribed by Maria H. Kleemiß; Martin Gilges; Prof. Gerhard Schomburg

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
793 KB
Volume
14
Category
Article
ISSN
0173-0835

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## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m

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Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g