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Capillary electrophoresis of DNA in entangled polymer solutions

✍ Scribed by Paul D. Grossman; David S. Soane

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
621 KB
Volume
559
Category
Article
ISSN
1873-3778

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πŸ“œ SIMILAR VOLUMES

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## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m

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Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g