Capillary electrophoresis-mass spectrometry methods for tryptic peptide mapping of therapeutic antibodies
β Scribed by Colin D. Whitmore; Lynn A. Gennaro
- Book ID
- 112083397
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 769 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0173-0835
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We have established a method for separation and characterization of a series of peptide hormones of pharmaceutical interest and wide therapeutical use by capillary electrophoresis-electrospray-mass spectrometry (CE-ES-MS) using a sheath flow interface. Several parameters were systematically investig
Capillary electrophoresis/electrospray ionization (CE/ESI) high mass accuracy time-of-flight mass spectrometry was used for the first time to characterize small proteins using peptide mapping. To identify small proteins, the intact proteins were first analyzed to obtain their average molecular weigh