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Can DNA polymerase I (Klenow fragment) serve as a model for other polymerases?

โœ Scribed by Joyce, Catherine M.


Book ID
123025449
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
593 KB
Volume
1
Category
Article
ISSN
0959-440X

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A domain of the klenow fragment of Esche
โœ P. S. Freemont; Dr. D. L. Ollis; T. A. Steitz; C. M. Joyce ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 868 KB

The Klenow fragment of DNA polymerase I from Escherichia coli has two enzymatic activities: DNA polymerase and 3'-5' exonuclease. The crystal structure showed that the fragment is folded into two distinct domains. The smaller domain has a binding site for deoxynucleoside monophosphate and a divalent