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NaF and mononucleotides as inhibitors of 3'-5'-exonuclease activity and stimulators of polymerase activity of E. coli DNA polymerase I Klenow fragment

✍ Scribed by I.A. Potapova; G.A. Nevinsky; V.V. Khomov; O.I. Lavrik


Book ID
115923631
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
348 KB
Volume
277
Category
Article
ISSN
0014-5793

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The Klenow fragment of DNA polymerase I from Escherichia coli has two enzymatic activities: DNA polymerase and 3'-5' exonuclease. The crystal structure showed that the fragment is folded into two distinct domains. The smaller domain has a binding site for deoxynucleoside monophosphate and a divalent