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Caffeine enhancement of radiation killing in different strains of Saccharomyces cerevisiae

✍ Scribed by Hannan, M. A. ;Nasim, A.

Publisher
Springer
Year
1977
Tongue
English
Weight
540 KB
Volume
158
Category
Article
ISSN
0026-8925

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✦ Synopsis

Haploid and diploid wild type strains, and three classes of radiation-sensitive mutants of Saccharomyces cerevisiae were tested for enhancement of UV-inactivation by caffeine in growth medium. In addition, the sensitizing effect of caffeine was studied in a haploid and a diploid wild type strain after gamma-irradiation. The drug sensitized the UV-irradiated cells of all strains except those reported to be only slightly UV-sensitive but highly sensitive to ionizing radiation. After gamma-irradiation, no caffeine-enhancement of killing was observed in stationary phase cells of either the haploid or the diploid strain. However, log-phase cells of both strains were partially sensitized. The results of both sets of experiments suggested that caffeine interferes with a recombinational repair occurring in cells in S or G2 phase.

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