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Cadmium induced metallothionein in hepatopancreas of Procambarus clarkii: Quantification by a silver-saturation method

✍ Scribed by Manuela Martínez; Amparo Torreblanca; Jose Del Ramo; Agustín Pastor; Javier Díaz-Mayans


Book ID
113380736
Publisher
Elsevier Science
Year
1993
Weight
537 KB
Volume
105
Category
Article
ISSN
0306-4492

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Quantification of Oxidized Metallothione
✍ D. Klein; S. Sato; K.H. Summer 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 387 KB

A rapid and easy to perform method for determining oxidized metallothionein (MT) is described. The main features of the procedure are that oxidized MT is converted into native MT with 2 -mercaptoethanol as reducing agent and \(\mathrm{Zn}^{2+}\) as metal donor, and MT is subsequently quantifled via