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Determination of metallothionein in tissues by radioimmunoassay and by cadmium saturation method

✍ Scribed by Canice V. Nolan; Zahir A. Shaikh

Book ID
107714356
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
902 KB
Volume
154
Category
Article
ISSN
0003-2697

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Quantification of Oxidized Metallothione
Quantification of Oxidized Metallothionein in Biological Material by a Cd Saturation Method
✍ D. Klein; S. Sato; K.H. Summer πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 387 KB

A rapid and easy to perform method for determining oxidized metallothionein (MT) is described. The main features of the procedure are that oxidized MT is converted into native MT with 2 -mercaptoethanol as reducing agent and \(\mathrm{Zn}^{2+}\) as metal donor, and MT is subsequently quantifled via