Quantification of metallothioneins by a silver-saturation method
β Scribed by A.M. Scheuhammer; M.George Cherian
- Book ID
- 113206757
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 778 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0041-008X
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π SIMILAR VOLUMES
A rapid and easy to perform method for determining oxidized metallothionein (MT) is described. The main features of the procedure are that oxidized MT is converted into native MT with 2 -mercaptoethanol as reducing agent and \(\mathrm{Zn}^{2+}\) as metal donor, and MT is subsequently quantifled via
A rapid and sensitive method for determining Cu-containing metallothionein (MT) is described. The main features of this Cd-saturation assay are: high-molecular-weight Cd-binding compounds are denatured with acetonitrile (50% final concentration), Cu bound to MT is removed with ammonium tetrathiomoly