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BurrH: a new modular DNA binding protein for genome engineering

✍ Scribed by Juillerat, Alexandre; Bertonati, Claudia; Dubois, Gwendoline; Guyot, Valérie; Thomas, Séverine; Valton, Julien; Beurdeley, Marine; Silva, George H.; Daboussi, Fayza; Duchateau, Philippe


Book ID
126861545
Publisher
Springer Science and Business Media LLC
Year
2014
Tongue
English
Weight
762 KB
Volume
4
Category
Article
ISSN
2045-2322

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We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein