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CBM21 starch-binding domain: A new purification tag for recombinant protein engineering

✍ Scribed by Shu-Chuan Lin; I-Ping Lin; Wei-I Chou; Chen-An Hsieh; Shi-Hwei Liu; Rong-Yuan Huang; Chia-Chin Sheu; Margaret Dah-Tsyr Chang

Book ID
116828953
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
417 KB
Volume
65
Category
Article
ISSN
1046-5928

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## Abstract The choline‐binding domain (ChoBD) of the carboxy‐terminal region of the __Streptococcus pneumoniae__ amidase LYTA (C‐LYTA) presents a strong affinity for tertiary amines. We report a method for single‐step purification of proteins expressed in the methylotrophic yeast __Pichia pastoris