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A novel approach for purification of recombinant proteins using the dextran-binding domain

โœ Scribed by Kuniyoshi Kaseda; Takao Kodama; Kazuhiro Fukui; Keiko Hirose

Book ID
117103153
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
152 KB
Volume
500
Category
Article
ISSN
0014-5793

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## Abstract The cholineโ€binding domain (ChoBD) of the carboxyโ€terminal region of the __Streptococcus pneumoniae__ amidase LYTA (Cโ€LYTA) presents a strong affinity for tertiary amines. We report a method for singleโ€step purification of proteins expressed in the methylotrophic yeast __Pichia pastoris