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Evaluation of a Modular Strategy for the Construction of Novel Polydactyl Zinc Finger DNA-Binding Proteins †

✍ Scribed by Segal, David J.; Beerli, Roger R.; Blancafort, Pilar; Dreier, Birgit; Effertz, Karin; Huber, Adrian; Koksch, Beate; Lund, Caren V.; Magnenat, Laurent; Valente, David; Barbas, Carlos F.

Book ID: 120436584
Publisher: American Chemical Society
Year: 2003
Tongue: English
Weight: 314 KB
Volume: 42
Category: Article
ISSN: 0006-2960
DOI: 10.1021/bi026806o

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## Abstract A peptide corresponding to the three zinc finger domains of the human transcription factor Sp1 has been expressed and found to bind a consensus Sp1 binding site with the sequence 5′‐GGGGCGGGG‐3′. Examination of the amino acid distributions within a large zinc finger sequence data base a