Blue Native electrophoresis to study mitochondrial and other protein complexes
✍ Scribed by Leo G.J Nijtmans; Nadine S Henderson; Ian J Holt
- Book ID
- 117702073
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 205 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1046-2023
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📜 SIMILAR VOLUMES
The nucleus is a highly structured organelle with distinct compartmentalization of specific functions. To understand the functions of these nuclear compartments, detailed description of protein complexes which form these structures is of crucial importance. We explored therefore the potential of blu
## Abstract Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on