The nucleus is a highly structured organelle with distinct compartmentalization of specific functions. To understand the functions of these nuclear compartments, detailed description of protein complexes which form these structures is of crucial importance. We explored therefore the potential of blu
Modified native electrophoresis protocol for the solubilization and separation of mitochondrial protein complexes
β Scribed by Elizabeth Sousa da Cunha; Cleyton Crepaldi Domingues; Eneida de Paula
- Book ID
- 113412162
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 222 KB
- Volume
- 418
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Blue native polyacrylamide gel electrophoresis ers for BN-PAGE, induces a charge shift on the proteins (BN-PAGE) or colorless native polyacrylamide gel so that the electrophoretic mobility of the proteins is electrophoresis (CN-PAGE) allowed separation of the mainly determined by the negative charge
The walls of fused-silica capillaries were chemically modified with small carbohydrate moieties in order to diminish the wall adsorption of proteins in capillary zone electrophoresis. A diol-type coating, prepared by bonding of gamma-glycidoxy propyltrimethoxysilane to the wall followed by acidic hy