Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue-native polyacrylamide gel electrophoresis
β Scribed by Mohammed Sabar; Janneke Balk; Christopher J. Leaver
- Book ID
- 110867342
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 284 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0960-7412
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Mitochondrial dysfunction and degeneration are associated with many neurodegenerative disorders. A dysfunctional mitochondrial electron transport chain (ETC) impairs ATP production and accelerates the generation of free radicals. To evaluate mitochondrial function, reliable methods are needed. Conve
Blue native polyacrylamide gel electrophoresis ers for BN-PAGE, induces a charge shift on the proteins (BN-PAGE) or colorless native polyacrylamide gel so that the electrophoretic mobility of the proteins is electrophoresis (CN-PAGE) allowed separation of the mainly determined by the negative charge