Biosynthesis of iodinin: Incorporation of [6-14C]shikimic acid
β Scribed by R.B. Herbert; F.G. Holliman; P.N. Ibberson
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- French
- Weight
- 185 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
The role of shikimic acid (1) as precursor for a number of antibiotic phenazines' has been established among others for iodinin (2). Our previous results" have led to the conclusion that for iodinin the possible pairing schemes of two shikimic acid molecules can be narrowed down to schemes A and B.
## Abstract Shikimicβ6β^13^C acid, 43 Β± 5% enriched, was synthesized from 91% enriched aceticβ2β^13^C acid via 91% enriched phosphoenolpyruvicβ3β^13^c acid. The latter intermediate was condensed with erythroseβ4βphosphate by a cell free extract of __E__. __coli__ 83β24.
## Abstract A convenient and inexpensive method for the preparation of [^14^C]βlabelled shikimic acid based on the photoassimilation of ^14^CO~2~ by henbane (__Hyoscyamus niger L.__) leaves in the presence of the herbicide glyphosate is described. Methanolic extracts were purified by successive an