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Biosynthesis of glucose-d-13C6 from Hansenula polymorpha and methanol-13C

✍ Scribed by Zhan-feng Liu; Zheng Ren; Da-gang Xu; Liang-jun Li

Publisher
John Wiley and Sons
Year
2011
Tongue
French
Weight
267 KB
Volume
55
Category
Article
ISSN
0022-2135

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✦ Synopsis

A simple and effective method for synthesis of glucose‐d‐^13^C~6~ by fermentation using the methylotrophic yeast Hansenula polymorpha with 99% abundance methanol‐^13^C is described. Using methanol‐^13^C as a sole source of carbon, H. polymorpha can accumulate large amounts of α,α‐trehalose‐^13^C~12~ under unfavourable growth conditions; the trehalose can then be hydrolysed to give glucose‐d‐^13^C~6~ with 98.5% abundance ^13^C. Copyright © 2011 John Wiley & Sons, Ltd.

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