Biosensor analysis of the interaction of potential dimerization inhibitors with HIV-1 protease
β Scribed by P. V. Ershov; O. V. Gnedenko; A. A. Molnar; A. V. Lisitsa; A. S. Ivanov; A. I. Archakov
- Book ID
- 111472127
- Publisher
- Pleiades Publishing
- Year
- 2009
- Tongue
- English
- Weight
- 347 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1990-7508
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The interaction between HIV-1 protease and reversible inhibitors was studied by surface plasmon resonance biosensor technology. The steady-state binding level and the time course of association and dissociation could be observed by measuring the binding of inhibitors injected in a continuous flow of
The interaction between HIV-1 protease and inhibitors has been studied with optical biosensor technology. Optimized experimental procedures and mathematical analysis permitted determination of association and dissociation rate constants. A sensor surface with native enzyme was unstable and exhibited