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Bioluminescent immunoassay for α-fetoprotein

✍ Scribed by Béatrice Térouanne; Jean-Claude Nicolas; André Crastes de Paulet

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
445 KB
Volume
154
Category
Article
ISSN
0003-2697

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✦ Synopsis

A bioluminescent immunoassay of n-fetoprotein is described. It uses monoclonal antibodies labeled with glucose-6-phosphate dehydrogenase and polyclonal antibodies coimmobilized on Sepharose with bioluminescent enzymes from marine bacteria, The bioluminescent reaction which occurs in the immunosorbent is proportional to the amount of cu-fetoprotein in the assay. The protocol is simple and rapid, and no separation step is required to remove the excess labeled antibodies. The assay can be performed directly on 25 ~1 serum and it is as sensitive as other immunometric assays. 'a I986 Academtc Press. Inc.

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