Immobilized anti-α-fetoprotein discs prepared by radiation polymerization for enzyme immunoassay
✍ Scribed by Minoru Kumakura; Isao Kaetsu; Mieko Suzuki; Shoichi Adachi; Kenichi Imagawawa
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 407 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Immobilized anti-cY-fetoprotein discs for enzyme immunoassay of Lu-fetoprotein were prepared by radiation polymerization of hydroxyethylmethacrylate at -78°C. The activity (determined by absorbance at 492 nm) of the discs varied with monomer concentration and disc thickness, the optimum values being 200-400 g 1-l and lo-30 pm, respectively. The discs have a porous polymer matrix, are flexible, and can be dried and stored for long periods. The correlation coefficient for a-fetoprotein concentration determined in sera by radioimmunoassay and by enzyme immunoassay with the discs was high (0.97). The recovery of ol-fetoprotein added to serum was 99.0-105%.