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Application of a mimetic enzyme for the enzyme immunoassay for α-1-fetoprotein

✍ Scribed by Ci Yun-Xiang; Qin Yang; Chang Wen-Bao; Li Yuan-Zong

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
295 KB
Volume
300
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

A new mimetic enzyme immunoassay (MEIA) method for cY-1-fetoprotein (AFP) was developed using a mimetic enzyme Mn( III)-tetra( suIfophenyl)porphine (Mn-TPPS,) as a labelling reagent to catalyze the fluorescence reaction of 4-hydroxyphenylacetic acid (HPA) and hydrogen peroxide. In the sandwich assay standard AFP solution or AFP containing umbilical blood serum Crst reacts with antibody (anti-AFP) coated on a 40-well plate (polystyrene), and then further reacts with Mn-TPPS,-labelled anti-AFP; the Mn-TPP& on the bound fraction, after removing the free fraction was determined by measuring the fluorescence intensity as a result of the reaction between HPA and H202, catalyzed by bound Mn-TPPS, and anti-AFP conjugate, which was proportional to the concentration of AFP. AFT in the range 0.01-10 pg per well can be determined with a detection limit of 1 ng per well. The method has sufficient sensitivity to be applied to the determination of AFP in umbilical blood serum.

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