๐”– Bobbio Scriptorium
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Bioluminescent assays of picomole levels of various metabolites using immobilized enzymes

โœ Scribed by G. Wienhausen; M. DeLuca

Book ID
102984602
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
687 KB
Volume
127
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Beneckea harveyi have been coimmobilized onto Sepharose 4B along with various other enzymes. By using the appropriate enzymes, bioluminescent assays have been developed for the following metabolites: Dghtcose, L-lactate, 6-P-ghtconate, L-malate, L-alanine, L-glutamate, NAD, and NADP. The optimal conditions for each assay have been defined. The lower limits of detection are different for each metabolite; in general, however, lo-100 pmol can be measured. Greatest sensitivity was observed with NADP, 0.2 pmol, and NAD, I pmol. The assays are rapid, reproducible, and exhibit a large concentration range in which light output is linear with respect to metabolite concentration.

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