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Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay

โœ Scribed by Richard Cathcart; Elizabeth Schwiers; Bruce N. Ames

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
430 KB
Volume
134
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A highly sensitive fluorometric method for the quantitation of cholesterol, lipid, and other hydroperoxides at the picomole level is described. The method is based on the oxidation of dichlorofluoroscin to the fluorescent dichlorofluoroscein by hydroperoxide and hematin under mild conditions. A 1:1 stoichiometry is observed between the hydroperoxide added and the dichlorofluoroscein produced. Since endoperoxides (e.g., PGH2) do not react in the assay, they do not interfere in the determination of lipid hydroperoxides.

๐Ÿ“œ SIMILAR VOLUMES

Determination of picomole quantitites of
Determination of picomole quantitites of hydroperoxides by a coupled glutathione peroxidase and glutathione disulfide specific glutathione reductase assay
โœ Kenneth G.D. Allen; Ching-Jang Huang; Catherine L. Morin ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 477 KB

A procedure to quantitate picomole amounts of hydroperoxides based on GSSG formation is described. Hydroperoxides are incubated with GSH and glutathione peroxidase, and the GSSG formed is measured by a GSSG-specific glutathione reductase recycling assay. Prior to analysis the remaining GSH is remove