✦ LIBER ✦
Bioluminescent microassay of various metabolites using bacterial luciferase co-immobilized with multienzyme systems
✍ Scribed by N.N. Ugarova; O.V. Lebedeva; I.G. Frumkina
- Book ID
- 102985032
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 550 KB
- Volume
- 173
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Co-immobilization methods have been developed for a bienzymatic system of luminescent Beneckea harveyi bacteria with formate dehydrogenase, glucose-6-phosphate dehydrogenase, and phosphoglucomutase. Bioluminescent assays have been devised for NADH, NAD, FMN, glucose 6-phosphate, and glucose 1-phosphate using the co-immobilized enzyme preparation. The lowest detection limits were in the picomole range with the bacterial extract and in the femtomole range with the partially purified enzymes, bacterial luciferase, and NADH:FMN oxidoreductase.