Biological activity and separation of a leukaemogenic virus from murine sarcoma virus-harvey (MSV-H)

✍ Scribed by Jennifer J. Harvey; June East

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: French
• Weight: 785 KB
• Volume: 4
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910040511

✦ Synopsis

Twelve diflerent preparations of Murine Sarcoma Virus-Harvey ( M S V -H ) had leukaemogenic activity when tested in BALBIc and C,H/Bi mice or Sprague-Dawley rats, some of which ultimately developed generalized lymphocytic leukaemia instead of reacting rapidly and characteristically with sarcoma formation andlor erythroblastic splenomegaly. Inactivation of MS V-H by ether treatment, reducing its titre by dilution, and using the intramuscular route of injection, all biased towards leukaernogenesis. The leukaemogenic component could be separated by vertical transmission from thret M S V-H-infected female mice, which themselves showed only sarcomas andlor splenic erythroblastosis, to as many as six successive generations of their untreated offspring. Presence of a leukaemogenic virus, probably M L V , was confirmed by serial passage of plasma filtrates from leukaemic animals, including leukaemic progeny, in newborn mice and rats. Lymphocytic leukaemia developed exclusively in these recipients and in those given a plasma concentrate. Moreover, pre-treatment with the leukaemogenic virus enhanced the oncogenicity of MSV-H in 8-and 14-day-old mice, as judged by an increased incidence of sarcomas. Pathological changes in the kidneys of the leukaernic mice are also described, which probably represent an immune deposit lesion related to the deposition of circulating complexes of viral antigen and anti-viral antibody on the basement membranes of the glomerular capillaries.