𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Bioassay detection of mouse nerve growth factor (mNGF) in the brain of adult mice

✍ Scribed by S. M. Scott; R. Tarris; D. Eveleth; H. Mansfield; M. E. Weichsel Jr.; D. A. Fisher

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
331 KB
Volume
6
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Two pools of seven brains each from adult Swiss‐Webster mice were homogenized, and supernatants were collected for bioassay. PC‐12 cells were placed in a bioassay plate at time zero, at a concentration of 10^4^ cells per well, and primed for 48 hours in a medium containing 50 ng/ml of mNGF. The PC‐12 cell bioassay for neurite outgrowth was conducted after primed cells were exposed to an NGF‐free medium for 24 hours. Suitable controls for serum toxicity and cell viability were established. The sensitivity of the bioassay approximates 100 pg NGF/ml. The results showed 80–100% neurite outgrowth in wells exposed to brain pool supernatant (BPS) alone, and control level outgrowth (3–8%) in wells containing BPS and specific anti‐β‐NGF antibody. Therefore, the brains of Swiss‐Webster adult mice contain an NGF‐like substance which promotes neurite outgrowth in PC‐12 cells. The substance probably is NGF itself, since the effect is blocked by specific NGF antiserum.

📜 SIMILAR VOLUMES

Sympathetic axons invade the brains of m
Sympathetic axons invade the brains of mice overexpressing nerve growth factor
✍ Kawaja, Michael D.; Crutcher, Keith A. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 821 KB

Transgenic mice that overexpress nerve growth factor (NGF) in cells producing glial fibrillary acidic protein were used to determine whether sympathetic axons will invade the undamaged, postnatal mammalian brain. By using reverse transcriptase-polymerase chain reaction, NGF mRNA transgene expression

Brain-derived nerve growth factor in the
Brain-derived nerve growth factor in the treatment of sensorineural hearing loss
✍ Eric Meen; Brian Blakley; Taeed Quddusi 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB

## Abstract ## Objectives/Hypothesis: A possible medical treatment for sensorineural hearing loss using brain‐derived nerve growth factor (BDNF) was explored. The hypothesis is that direct intracochlear application of BDNF will result in improved hearing. ## Study Design: Animal research study.

Microsphere embolism-induced elevation o
Microsphere embolism-induced elevation of nerve growth factor level and appearance of nerve growth factor immunoreactivity in activated T-lymphocytes in the rat brain
✍ Hideyuki Mizuma; Kaori Takagi; Keiko Miyake; Norio Takagi; Kumi Ishida; Satoshi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB 👁 2 views

Changes in nerve growth factor (NGF) level and type of cells producing NGF were investigated in the rat brain after sustained cerebral embolism. The NGF level was determined by a two-site enzyme immunoassay specific for NGF. The cerebral cortex, striatum, and hippocampus of the embolized hemisphere

Effects of the neurotrophins nerve growt
Effects of the neurotrophins nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor (BDNF) on neurite growth from adult sensory neurons in compartmented cultures
✍ Kimpinski, K. ;Campenot, R. B. ;Mearow, K. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 299 KB 👁 2 views

We used compartmented cultures into NGF plus NT3 compartments, while the combinato study the regulation of adult sensory neurite growth tion of BDNF plus NGF resulted in an inhibition of by neurotrophins. We examined the effects of the neuneurite extension compared with NGF alone. We then rotrophins

Age-dependent time course of cerebral br
Age-dependent time course of cerebral brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 in APP23 transgenic mice
✍ O. Schulte-Herbrüggen; S. Eckart; U. Deicke; A. Kühl; U. Otten; H. Danker-Hopfe; 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 355 KB

## Abstract Neurotrophins, including brain‐derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin‐3 (NT‐3), have repeatedly been shown to be involved in the pathophysiology of Alzheimer's disease (AD). Recent studies have claimed that these neurotrophic factors are importan

Increase of a nerve growth factor-like p
Increase of a nerve growth factor-like protein in the cerebellum of PCD mutant mice
✍ Joan P. Schwartz; Bernardino Ghetti; Lewis Truex; Michael J. Schmidt 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 437 KB

## Abstract Several areas of mouse brain (cerebellum, brain stem, and hemispheres) contain levels of nerve growth factor (NGF) detectable by radioimmunoassay. However, this immunoactive NGF is not biologically active in the chick embryo sensory ganglia bioassay. Cerebellum from pcd mice, in which n