Effects of the neurotrophins nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor (BDNF) on neurite growth from adult sensory neurons in compartmented cultures
✍ Scribed by Kimpinski, K. ;Campenot, R. B. ;Mearow, K.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 299 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We used compartmented cultures into NGF plus NT3 compartments, while the combinato study the regulation of adult sensory neurite growth tion of BDNF plus NGF resulted in an inhibition of by neurotrophins. We examined the effects of the neuneurite extension compared with NGF alone. We then rotrophins nerve growth factor (NGF), neurotrophininvestigated whether the regrowth of neurites that had 3 (NT3), and BDNF on distal neurite elongation from originally grown into NGF subsequent to in vitro axoadult rat dorsal root ganglion (DRG) neurons. Neutomy still required NGF. The results demonstrated rons were plated in the center compartments of threethat unlike adult sensory nerve regeneration in vivo, chambered dishes in the absence of neurotrophin, and the in vitro regrowth did require NGF, and neither neurite extension into the distal (side) compartments BDNF nor NT3 was able to substitute for NGF. Since containing NGF, BDNF, or NT3 was quantitated. Ini-
the initial growth from neurons after dissociation tial proximal neurite growth did not require any of (which is also a regenerative response) did not require the neurotrophins, while subsequent elongation into NGF, it would appear that neuritic growth and redistal compartments required NGF. After neurites growth of adult DRG neurons in vitro includes both had extended into NGF-containing distal compart-NGF-independent and NGF-dependent components. ments, removal of NGF by treatment with anti-NGF The compartmented culture system provides a unique resulted in the cessation of growth with minimal neumodel to further study aspects of this differential regrite retraction. In contrast to the effects of NGF, no ulation of neurite growth.