𝔖 Bobbio Scriptorium
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Binding of lactate dehydrogenase isozyme X (LDH-X) by sperm antibodies of infertile humans

✍ Scribed by M. Coughlan; B. Boettcher; T. Roberts


Book ID
118376051
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
59 KB
Volume
8
Category
Article
ISSN
0093-691X

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A comparative study of catalytic properties of the sperm-specific lactate dehydrogenase (EC 1.1.1.27) isozyme X or C4 from a variety of animals (boar, bull, goat, Guinea pig, man, mouse, pigeon, rabbit, and rat) is presented. Optimum concentration and Km values for pyruvate, inhibition by substrate,

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## Abstract LDH‐X from spermatozoa can be demonstrated within the perivitelline space and zona pellucida of the preimplantation mouse embryo. Peroxidase reaction product is deposited in these areas of blastocysts incubated with rabbit anti‐LDH‐X IgG and then with peroxidase labelled goat anti‐rabbi