Binding of acridine orange to yeast ribosomes
β Scribed by Morgan, Richard S.; Rhoads, David G.
- Book ID
- 123191556
- Publisher
- Elsevier Science
- Year
- 1965
- Weight
- 159 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0926-6585
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## Abstract Exposure of __Escherichia coli__ MREβ600 ribosomes to acridine orange (AO) at low ionic strength (1m__M__ Trisβacetate pH 7.4) results in quantitative binding of the dye. Under our experimental conditions about a few hundred dye molecules can be bound to any one of the 30, 50, or 70βS p
In hippocampal neurons of ground squirrels and neuroblastoma culture the ribosome state was analyzed by staining with acridine orange (AO), labeling with radioactive amino acids, and electron microscopy. Electron microscopy indicated that the extent to which ribosomes associated in polysomes varied