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The effect of acridine orange on the structure of ribosomes

✍ Scribed by Moshe Tal; Haim Rotem; Matya Alfasi; Robert A. Berg

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
350 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Exposure of Escherichia coli MRE‐600 ribosomes to acridine orange (AO) at low ionic strength (1m__M__ Tris‐acetate pH 7.4) results in quantitative binding of the dye. Under our experimental conditions about a few hundred dye molecules can be bound to any one of the 30, 50, or 70‐S particles. AO causes the 30 and the 50‐S subunits to form ribosomal aggregates of approximate sedimentation constants of 70 and 100‐S.

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