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Magnesium binding to yeast ribosomes

✍ Scribed by J. A. L. I. Walters; G. A. J. Van Os


Book ID
102763436
Publisher
Wiley (John Wiley & Sons)
Year
1971
Tongue
English
Weight
510 KB
Volume
10
Category
Article
ISSN
0006-3525

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## Abstract Proflavine binding experiments were carried out with yeast rRNA, native and β€œunfolded” ribosomes; the binding constants and the number of binding sites were calculated by a spectroscopic method. The study of the intercalation complexes by fluorescence and electric dichroism shows the in

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Rat liver ribosomes and polyribosomes could be immobilized in agarose gels at 4Β°C and pH 7.6, using KC1 or NaCl molarities of 0.25 or higher. The binding could be effected in the presence of excess protein and/or detergents. Polysomes attached to endoplasmic membrane fragments did not bind to agaros