Isothermal titration calorimetry is a high-accuracy method for measuring binding affinities. Titration calorimetry is a universal method that has broad impact throughout biotechnology. In recent years, microcalorimeters that are capable of characterizing binding interactions of biological macromolec
Binding isotherm determination by isothermal titration calorimetry
โ Scribed by A. A. Saboury
- Book ID
- 111597031
- Publisher
- Springer Netherlands
- Year
- 2004
- Tongue
- English
- Weight
- 132 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0022-5215
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๐ SIMILAR VOLUMES
A rigorous method for the least-squares nonlinear regression analysis of displacement isothermal titration calorimetric data is presented. The method can fit the binding isotherm of a ligand which is competitively inhibited in its binding by another bound ligand to a molecule with n identical and in
The construction of an automatic gradient titration apparatus using a multichannel peristaltic pump and a recording spectrophotometer is described. The ability of the apparatus to faithfully generate continuous spectrophotometric binding isotherms was tested in experiments studying the interaction o