Isothermal titration calorimetry is a high-accuracy method for measuring binding affinities. Titration calorimetry is a universal method that has broad impact throughout biotechnology. In recent years, microcalorimeters that are capable of characterizing binding interactions of biological macromolec
Thermodynamic Analysis of Chitooligosaccharide Binding toUrtica dioicaagglutinin by Isothermal Titration Calorimetry
β Scribed by Samiksha Katiyar; E. J. M. Van Damme; Willy J. Peumans; Avadhesha Surolia
- Book ID
- 110394004
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 494 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0144-8463
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A rigorous method for the least-squares nonlinear regression analysis of displacement isothermal titration calorimetric data is presented. The method can fit the binding isotherm of a ligand which is competitively inhibited in its binding by another bound ligand to a molecule with n identical and in
## Abstract The serine proteinase inhibitor antithrombin III (ATIII) is a key regulatory protein of intrinsic blood coagulation. ATIII attains its full biological activity only upon binding polysulfated oligosaccharides, such as heparin. A series of synthetic peptides have been prepared based on th