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Exact Analysis of Competition Ligand Binding by Displacement Isothermal Titration Calorimetry

✍ Scribed by Bent W. Sigurskjold

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
291 KB
Volume
277
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rigorous method for the least-squares nonlinear regression analysis of displacement isothermal titration calorimetric data is presented. The method can fit the binding isotherm of a ligand which is competitively inhibited in its binding by another bound ligand to a molecule with n identical and independent binding sites. There are no other assumptions for the method and no approximations. Analysis of previously published data of the strong binding of acarbose to glucoamylase is presented as an example. The regression equations have been programmed for the Origin software supplied with the widely used titration calorimeters from Microcal, Inc., and an Origin Function Definition File with instructions is freely available from the author upon e-mail request.

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