Binding and redistribution of surface membrane receptors for concanavalin A on oyster hemocytes

✍ Scribed by Yoshino, Timothy P. ;Renwrantz, Lothar R. ;Cheng, Thomas C.

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 891 KB
• Volume: 207
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402070312

✦ Synopsis

Abstract

The binding and redistribution of Con A surface membrane determinants was investigated in two Crassostrea virginica hemocyte subpopulations (designated LS and SS) by employing a direct Con A‐peroxidase coupling method in conjunction with light and electron microscopical cytochemistry. At 4°C small, adherent hemocytes exposed to Con A and horseradish peroxidase (HPO) produced a uniform staining pattern on hemocyte surface membranes. Polar redistribution or capping of Con A receptors was observed in 22.2% of LS and 11.0% of SS hemocytes when the incubating temperature was increased from 4 to 21°C. Receptor patching, observed with equal frequency (∼15%) in both cell subpopulations, also exhibited a temperature dependency. Capped hemocytes were readily identified by the polar concentration of darkstaining HPO reaction product along the marginal surface of each cell. Receptor patching was observed as multiple aggregates of reaction staining on blood cell surface membranes. Cap formation in SS hemocytes was inhibited by sodium azide (^−^N~3~) at each concentration of inhibitor tested. LS hemocyte capping was inhibited only at the highest ^−^N~3~ concentration (5 × 10^−2^ M). Cells of both subpopulations were unable to completely reverse the effects of ^−^N~3~ poisoning following inhibitor removal.