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Bile acid-controlled transgene expression in mammalian cells and mice

✍ Scribed by Rössger, Katrin; Charpin-El-Hamri, Ghislaine; Fussenegger, Martin


Book ID
123246125
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
709 KB
Volume
21
Category
Article
ISSN
1096-7176

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## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi